Method for liquid enrichment with oxygen and applications of enriched liquids

ABSTRACT

The present invention relates to a method for preparing oxygen enriched liquids by subjecting a liquid under hyperbaric oxygen conditions within an oxygen enrichment chamber of a sealed hyperbaric apparatus and maintaining the liquid under hyperbaric pressure in conditions and for a time sufficient to dissolve and stabilizing the oxygen in the liquid. The oxygen enriched liquids are suitable for use in cosmetic and medical field and for preparing a drinkable oxygen enriched beverage.

FIELD OF THE INVENTION

[0001] The present invention relates to oxygen enriched liquids, methodsfor making the same and their applications in the medical and cosmeticfields.

BACKGROUND OF THE INVENTION

[0002] It is known that mammals are not capable of making their ownoxygen reserve and therefore an oxygen deficiency in the body oftencauses tissues changes known as hypoxia, hypoxidosis and anoxia.

[0003] Transport of the oxygen is linked to blood vessels, erythrocytes,hemoglobin, iron and DPG. Since erythrocytes do not enter the cells,diffusion process occurs, enabling the penetration of oxygen through thecell membrane, causing the cell metabolism to function and creatingelectrical potential of the cells, as a manifestation of life of thosecells.

[0004] Every obstacle such as thrombi or injury that led tostrangulation or obstacle to the vessel circulation is preventing theoxygen to supply the tissue.

[0005] The deficiency of molecular oxygen in the tissue is imperilingthe tissue and pathological changes occur. These pathological changes inpathology and patho-physiology are classified from anemia to gangrene.

[0006] To date numerous methods have been developed to attempt toimprove oxygen delivery to tissues.

[0007] The law of physics of Daltons, Henry, Gay-Lussac, have provedthat every gas dissolves in liquids when put under pressure, which meansthat with higher pressure the solubility of the gas grows in the liquid.According to this principle, we have all the gasses dissolved in ourbody in liquid form.

[0008] Thus in the past numerous methods have been developed to improveoxygen delivery to tissues by means of inhalation through devices suchas tents, cannulas, masks, hoods and mechanical ventilator systems.

[0009] The purposes of oxygen inhalation is to increase the amount ofoxygen absorbed by erythrocytes to increase oxygen delivery to cells andtissues. However the inhalation systems of oxygen are associated withseveral drawbacks and complications.

[0010] In the attempt to overcome these limitations, Prof. Boerema etal. performed the usage of increased oxygen dissolved in liquids asdisclosed in “Life without blood: A study of the influence of highatmospheric pressure and hypothermia on dilution of blood”; J.Cardiovas. Surg.; 1, 133 1960. This article represents the beginning ofthe usage of oxygen under pressure or dissolved oxygen under pressure inall liquids of the human organism. The disclosed method of usage underpressure is widely spread today and it is used as a routine in thehyperbaric medicine. According to the disclosed practice, patientswithin a hyperbaric chamber inhale the oxygen through an oxygen maskunder the simulated conditions of depth.

[0011] If the obstacle of oxygen transport in the tissue exists, thisobstacle is superseded by the physically dissolved oxygen, both in thebigger blood vessel and in the microcirculatory net.

[0012] Being dissolved in all the liquids of the organism, physicallydissolved oxygen is being consumed 20% or 4 times more efficiently theninhaled through the mask in the hyperbaric chamber.

[0013] However, many limitations exists for these methods of oxygentherapy.

[0014] Firstly, inhaled in the hyperbaric chamber the consumption ofoxygen is only 5% and the increased blood oxygen levels achieved duringchamber pressurization are lost when the chamber is depressurized andthe patient is removed from the chamber.

[0015] Secondly, numerous complications have been associated with theuse of hyperbaric breathing chambers including fires, explosions, oxygentoxicity, gas-embolism and diseased caused by a rapid depressurization.

[0016] The occurrence of these drawbacks have led to limit the use ofhyperbaric chambers only to a restricted number of conditions.

[0017] Thus, suggestions have been made in the prior art to achieve analternative way to increasing the amount of oxygen available for aerobicand therapeutic processes.

[0018] Other methods of oxygen therapy involves the intravenousinjection of gaseous oxygen or the use of solutions oxygenated by meansof a complicate gas liquid contact apparatus. These methods have beenfound too hazardous or too complicated.

[0019] Thus recently have been developed some methodologies to obtain asufficiently high degree of oxygen enrichment of water.

[0020] It is also known to impregnate at elevated atmospheric pressurewith a gas from an external source a substantially gas-free liquid inorder to make the liquid effervescent. This artificial impregnation of aliquid is carried out by bubbling the gas through the liquid or byspraying the liquid into the gas or by violently agitating the liquid inthe presence of a gas.

[0021] However, these methods have not been found to be successful toobtain liquids at high rate of dissolved oxygen or to retain thedissolved oxygen in the liquid for a prolonged time.

[0022] U.S. Pat. No. 5,766,490 in the name of Taylor et al. discloses aprocess for inserting and dissolving oxygen in water in an oxygenenriching space by passing a liquid and the oxygen through static anddynamic turbulent mixers and recovering the oxygenated liquid.

[0023] A drawback of the disclosed process is that it requires acomplicated apparatus for enriching water with oxygen. In addition theproduced oxygen enriched water leads to bubbles appearance and thereforethe disclosed method is unsuitable to retain the dissolved oxygen in thewater for an appreciable length of time.

[0024] U.S. Pat. No. 5,814,222 in the name of Zelenák et al. describes amethod for enriching a liquid with oxygen and an apparatus including avessel provided with a number of horizontal trays vertically spacedapart from each other which allow to dissolve the supplied oxygen in aliquid. The liquids oxygenated by the disclosed apparatus are used invarious aerobic and therapeutic processes. The disclosed invention hasthe disadvantage that it is very difficult to maintain a desirable levelof cleanness and sterility of the disclosed complicated apparatus.Accordingly, the oxygenated liquids prepared with the disclosedinvention are not useful for medical purposes where strictlyrequirements of sterility are required.

[0025] EP 0 847 959 A1 discloses a method for producing liquids andbeverages enriched with oxygen by supplying oxygen under pressure to aliquid within a bottle prefilled with oxygen. The disclosed liquid isuseful only for the oral administration as oxygen integrator.

[0026] However, the oxygen incorporated in these liquid is not stableunder warm temperatures and tends to lose the additional oxygen loadingwhen the bottle are mixed such as during transport.

[0027] Thus, none of the known methodologies for increasing the oxygencontent in liquids have proved successful to achieve a sufficientlydegree of oxygenation of the liquid and to maintain this feature for anappreciable length of time.

[0028] Accordingly at present there is a need for increasing the contentof pure oxygen in water or liquids in general to achieve a high level ofdissolved oxygen and to retain the dissolved oxygen for a prolongedtime.

SUMMARY OF THE INVENTION

[0029] It is a principal aim of the present invention to provide amethod for producing an oxygen enriched liquid which retain thedissolved oxygen at high concentration for an appreciable length oftime.

[0030] Another object of the present invention is to enable productionof high oxygen levels dissolved in water with a good retention of thedissolved oxygen even after storage, at substantially reduced costs.

[0031] Yet another object of the present invention resides in providingtherapeutic or cosmetic methods of treatments employing physiologicalliquids enriched with oxygen in accordance with an aspect of the presentinvention.

[0032] A further object of the present invention is to provide a highlyoxygen enriched drinkable water or beverage useful as oxygendiet-supplement.

[0033] Still another object of the present invention is to provide amethod of oxygen therapy which is safe and avoids complications anddrawbacks associated with method of prior art.

[0034] Essentially, the method of oxygen enrichment according to a firstaspect of the present invention involves subjecting a liquid underhyperbaric and specific conditions for a time sufficient to achieve astable enriched oxygen liquid.

[0035] Thus, in accordance with a first aspect of the present inventiona method for enriching a liquid with oxygen is provided comprisingintroducing a liquid into a sealed container and dissolving oxygen insaid liquid under pressure and recovering the liquid enriched withoxygen.

[0036] As used throughout the specification and claims the term liquidis meant to include any liquid that is capable of being oxygenated underpressure such as water, physiological solutions, flavored or mineralwaters and liquid preparations for medical uses.

[0037] The method according to one aspect of the present invention doesnot impart any taste to the resulting liquid thus permitting the use ofthe water enriched with oxygen as a supplement liquid in the diet.

[0038] Typically, the sealed enriching container used in method of theinvention is mechanically cleaned and disinfected before the liquidtreatment. Advantageously, the enriching container is made of stainlesssteel and comprises a sealed chamber that supports high pressure such as150 atmospheres.

[0039] The oxygen dissolved in the liquid under pressure preferablyconsists of clean medical oxygen, a very pure oxygen used in the medicalfield.

[0040] In particular the use of 100% pure medical oxygen in accordancewith the pharmacopoeia provide oxygen enriched liquids useful for theadministration to humans in need of a therapeutic or prophylaxistreatment.

[0041] In accordance with an embodiment of the invention, the liquid tobe treated is firstly supplied into the sealed stainless steel chamberand then an oxygen flow is supplied in said chamber such that the oxygendissolves in the liquid. The interior of the chamber is put underpressure for producing a high oxygen loading of the liquid and to obtaina sufficiently high degree of oxygen enrichment of water which allows toretain the dissolved oxygen in the liquid for an appreciable length oftime under atmosphere pressure. Preferably the liquid is subjected tothe superoxidation procedure for a time between 10 and 90 minutes, morepreferably for a time between 50 and 70 minutes. After the expositiontime to high pressure is terminated, a decompression period begins untilthe atmosphere pressure is achieved. When the atmosphere pressure isachieved, then the oxygen enriched liquid can be taken out the chamberand collected in recipients for storage.

[0042] In accordance with an embodiment of the invention the liquidwithin the enrichment chamber is subjected to an oxygen pressure of 3 to40 atm, preferably of from 8 to 12 absolute atmospheres, convenientlyfor a time within the range of 45 to 80 minutes.

[0043] In these conditions, the process of the invention enables thedissolution of at least 30 mg/l oxygen in the liquid and the maintenanceof the achieved oxygen levels in the liquid for a prolonged time undercommon storage conditions.

[0044] In accordance with another embodiment of the present invention, amethod to enrich oxygen which allows to substantially avoid theformation of bubbles from the gas dissolved in said oxygen is providedin which oxygen is dissolved into a liquid preferably under a partialpressure of 3 to 10 Mpa (30-100 Bar) and then the oxygen enriched liquidis passed through capillary tubes.

[0045] Specifically, in order to prepare an oxygen enriched watersolution for endoarterial or endovenous administration, a sterilizedphysiological solution is enriched with oxygen in accordance with theabove disclosed methods and then said physiological solution isintroduced into capillary tubes preferably made of silicon and having adiameter between 60-90μ under a hydrostatic pressure which isconveniently selected between 50-60 Mpa in order to pass with greatvelocity through said capillary tubes.

[0046] In accordance with a preferred embodiment, a physiologicalsolution enriched with oxygen in accordance with the method of theinvention, is supplied into silicon capillaries under hydrostaticpressure of 50-70 Mpa in order to obtain a transit of 1 g/min with thespeed of 4 m/s through the capillary.

[0047] Liquid solutions which are useful for endovenous or intravenousadministration include physiological solutions, dextrose solution,Ringer or Hartmann solutions, perfluoro carbonic solutions and proteinsolutions such as PVP plasma and alike.

[0048] In accordance with another aspect, the present invention alsoprovides liquids enriched with oxygen obtained in accordance with theabove methods.

[0049] These oxygen enriched liquids may be used as such or in a formdiluted with other liquids. Suitably, these liquids have highlydissolved oxygen with standard partial pressure and with permanent bondsbetween the molecules of oxygen and hydrogen.

[0050] In particular, it has been found by performing depth chemicalbond analyses that in the oxygen enriched water of the invention themolecules form many hydrogen bonds, resulting in a structure thatresembles the structure of ice. These hydrogen bonds permit to achieve ahigh degree of oxygen enrichment of water and to retain the dissolvedoxygen for longer period of time than with the known methodologies. Inparticular, the molecules of hydrogen and oxygen in the oxygen enrichedwater according to the invention are very close to each other to providea stable molecular structure.

[0051] The oxygen enriched liquids of the invention are also useful intherapeutic methods of treatment of the human body or its parts, inconditions or diseases where an increased demand of oxygen is required.

[0052] Suitable therapeutic methods in which liquids made in accordancewith the present invention can be advantageously employed include allthe human conditions or pathologies where it is requested to increasethe oxygen content of blood and tissue.

[0053] The oxygen enriched liquids can be administered either assolutions per oral use or as solutions for internal use.

[0054] In particular, water enriched with oxygen according to theinvention may be orally administered for treating or preventinggastritis, ulcers, ulcus ventricle or duodena, duodenitis, colitisulcerosa and to the pathologies where lesions at the mucous membrane aremanifested. In these cases the direct application of the oxygen enrichedsolutions alleviates the symptoms and cures the damaged tissuespermitting an oxygen transfer.

[0055] Advantageously, sterile physiological solutions enriched withoxygen according to an embodiment of the invention can be orally orparentally administered in particular to prevent or treat all caseswhere degenerative changes to tissues occur such as in spondilosisdeforming, coxartrosis, Parkinson disease, Alzheimer disease,encephalomyelitis disseminant, stroke and other acute or chronicpathologies. In addition, in cases of vessel or circulatoryinsufficiency, and chronic long term damages or organs oxygen enrichedsolutions allow a more intensive metabolism, preventing the chronichypoxya that appeared for any reason through the course of time.

[0056] In accordance with another feature of the invention, solutionsenriched by oxygen of the invention can be intraarterially orintravenously administered in acute cases when the oxygen transport hasbeen suddenly interrupted by the occlusion of a primary artery, such asmyocardial infarct, stroke or cerebral infarct, occlusion of femoralartery. In fact in these conditions the oxygen deficiency at thediseased tissues is immediate and there is a request for a supplementaryoxygen supply.

[0057] When used intraarterially or intravenously, liquids enriched withoxygen under pressure according to an embodiment of the invention, areadministered using a cannula and a system for transfusionalapplications. These treatments are usually performed on the terminalartery of the organ or tissue in need of treatment. The same applies inthe case of the treatment of ulcer-varicose lesions or pelvicinflammatory processes.

[0058] In accordance with another embodiment, the oxygen enrichedsolutions made according to the method of the invention can be locallyadministered in the cases where the demand for oxygen is increased suchas in injuries at the epidermis in cases of burns, or local inflammatoryprocesses or in case of frostbites or in gynecology for example forsterility treatment and in traumatology for example in cases of bonefractures and damages of soft tissues.

[0059] In accordance to another embodiment, the degenerative changesconnected to biliary or liver system can be treated with the oxygenenriched solutions of the invention. With the oxygen treatment the zonesof local hypoxya that are a consequence of degenerative changes, arereduced and the damaged tissues regenerates faster. For example, incases of negative effects of radiotherapy, which are characterized by avascular net deficiency and blood vessels rigidity causing lowering ofblood flow, the infusion of an solution of an enriched physiologicalsolution of the invention permit to a reparation of the tissues damagedby irradiation, lower the connected pain and expands the movement range.

[0060] In accordance with yet another embodiment an oxygen enrichedliquid of the invention is suitable to treat or prevent the infectiousdiseases caused by viruses or bacteria such as the spore-formingBacillus anthracis by the topical or endovenous administration of aantibacterial effective amount of a physiological oxygen enrichedsolution made according to the invention. Advantageously, said oxygenenriched solution can also incorporate antibiotics or otheranti-infective agents suitable against bacterial infection. In thesecases the administration of the solution is advantageously made twice adays for a period of time necessary to wipe out the infection.

[0061] The oxygen enriched solutions of the invention were tested onexperimental animals subjected to an acute occlusion of blood vesselswhich provoked degenerative changes of the interested tissues.

[0062] An oxygen enriched physiological solution of 0.3 cc having anoxygen partial pressure of 2 bar was intravenously administered at arate of 1 g/min twice a days to a first group of 5 rats having amyocardial infarction due to a restriction of the coronaries. A secondgroup of rats with the same disease, was subjected to a conventionaloxygen treatment by inhalation by means of a cannula.

[0063] The results of the treatment were monitored and scheduled forfive days. The first group of animals, treated by administering asolution in accordance with an embodiment of the present inventionconsumed 20% of oxygen in the damaged tissues whereas the second groupwhich received the oxygen by inhalation consumed only 5% of oxygen inthe damaged tissues.

[0064] In accordance with another aspect of the present invention acosmetic treatment is provided comprising the application of acosmetically effective amount of an oxygen enriched liquid of theinvention or a composition including said liquid.

[0065] In particular the local or subcutaneous application on of asolution or a cosmetic preparation including a cosmetically amount of asolution of the invention, provides an additional source of oxygen tothe tissues in need of treatment, allowing a regeneration of damagedcells or tissues. In accordance with this aspect of the invention it ispossible to treat facial corrugations, wrinkles and other signs due toskin aging by the local or topical application of an oxygen enrichedsolution or of a cosmetic preparation including such composition.

[0066] For example, the enriched solution of the invention can beincorporated in a solid cosmetic vehicle or in liposomes to provideemulsions, creams or ointments for local applications one or twice aday. These cosmetic preparations can further include cosmetic activeingredients which act in conjunction with the oxygen source.

[0067] In accordance with another aspect of the present invention anoxygen enriched water is provided which can be used as a beverage inspite of potable water or as a supplement in a diet regimen whichrequires an additional source of oxygen supply such as in sportsmanlikeactivities.

[0068] In these cases the administration of 1 liter per day in thenormal dietetic regiment contributes to maintain an high efficiency ofthe principal physiological functions.

BRIEF DESCRIPTION OF THE DRAWINGS

[0069] The method of the present invention together with additionalobjects and advantages thereof will be best understood from thefollowing description of a specific, non limitative embodiment, whenread in conjunction with the accompanying drawings in which:

[0070]FIG. 1 is a side view of an hyperbaric apparatus for the liquidenrichment with oxygen according to an embodiment of the invention;

[0071]FIG. 2 is a lateral view taken from the other side of thehyperbaric apparatus shown in FIG. 1.

DETAILED DESCRIPTION OF THE INVENTION

[0072] Referring now to the drawings and first, particularly to FIG. 1thereof; with the generic number 1 is designed a cylindrical oxygenenrichment apparatus supported by two stands 13 and 14 and extendinghorizontally according to an embodiment of the invention.

[0073] A liquid to be treated according to the invention is lead atatmospheric pressure to an oxygen enrichment chamber 2 of a hyperbaricapparatus 1 through a supply tube 3 connected with a supply tank, notshown. The liquid is supplied by means of a pump, not shown, into theenrichment chamber 2 and the flow of liquid is controlled by means of aninlet valve 4. Said liquid can be water or physiological solutions orother water-based solutions which optionally are subjected to apreliminary purification and/or filtration.

[0074] Specifically, for medical uses the water to be treated accordingto an embodiment of the invention is disinfected or sterilized beforebeing subjected to the oxygenation treatment to avoid the use ofbacterial or viral contamination.

[0075] The enrichment chamber 2, which is conveniently made of stainlesssteel, is hermetically sealed during operative conditions and isengineered to support pressures higher than 100 atmospheres, in order toavoid the outflow of gases during oxygenation procedures or explosions.

[0076] An oxygen supplying tube 5, suitably provided with a check valve6 is joined to the enrichment chamber 2 for supplying an oxygen flowwithin the chamber 2. An Additional inlet 16 for supplying additionalgasses such as nitrogen, air or hydrogen is also provided in proximityof said supply tube 3.

[0077] The oxygen flow which is introduced into the chamber 2, can beobtained from many convenient sources and is stored in a conventionaloxygen vessel positioned in proximity of the chamber. The oxygen flowintroduced into the chamber 2 is regulated and controlled by the inletvalve 6 which permits to avoid a dangerous overpressure.

[0078] Suitably, a flow meter 7 can also be provided in connection withsaid oxygen supplying tube 5 to allow precise monitoring of thecontinuous inflow of the oxygen.

[0079] A manometer 8 is also provided as additional device to relieveand monitoring the interior pressure of the chamber during the procedureof liquid oxygenation. Suitably, the apparatus 1 is also provided with asafety valve 9 to avoid that the pressure within the chamber 2, raisesover dangerous levels.

[0080] As illustrated in FIG. 2, in accordance to an embodiment of theinvention a window 19 made of glass resistant to high temperature can beprovided on the lateral side of the hyperbaric apparatus 1.

[0081] Conveniently, a door 10 driven in by a bayonet can also beprovided on a lateral surface of the apparatus 1 to permit inspectionsof the internal chamber 2 and to allows specialized personal to enterthe hyperbaric chamber for the periodic mechanical cleaning of innersurfaces thereof. Suitably, the internal chamber 2 is smooth-surfaced toallow an easy mechanical and chemical sterilization of its innersurface.

[0082] Suitably, the amount of water supplied in the hyperbaric chamber2 does not exceed a third of the internal volume thereof. Conveniently,when the amount of supplied water reaches the desired volume, the inletvalve 6 is opened and an oxygen flow under pressure of about 11 absoluteatmospheres (11 bar) is introduced into the oxygen enrichment chamber 2.The oxygen is supplied until the internal oxygen pressure is of about 11atmospheres. These conditions are maintained for a time of about 60minutes during which the internal pressure is constantly metered by themanometer 8.

[0083] During the process of water oxygen enrichment performed athyperbaric conditions, it is also possible to check the rate of oxygendiffusion into the water by means of the manometer 8 and to control thestatus of the treatment by means of the window 19.

[0084] After the pressurization time of about 60 minutes a sufficientlyhigh degree of oxygen enrichment of water is achieved and the enrichedwater can be recovered after the chamber has been depressurized byflowing the oxygen atmosphere through the exhaust tube 17. After leavingthe enrichment chamber 2, the oxygen enriched water is forwarded throughthe outlets 12 and 15 to a bottling plant or to a unit storage notshown.

[0085] When the oxygenated water is stored in a bottle or in a sealedcontainer, the retention of the high level of oxygen is retained forprolonged times. This means that when the bottles or containers areopened and the enriched water consumed, the water retains close to itsinitial oxygen content during its consumption. Advantageously, beforesaid bottles or container are sealed the partial oxygen pressure ismeasured and the values should be the same as they were at the end ofthe oxygen enrichment treatment performed inside the hyperbaric chamber2.

[0086] In accordance with a preferred embodiment of the method of theinvention, physiological sterilized solutions, that are kept in closedcontainers such as vials for intravenous or intraarterialadministration, can be prepared and used for oxygenation purposes.

1. A method for enriching a liquid with oxygen comprising the steps of:introducing a liquid into an oxygen enriching chamber of a sealedhyperbaric apparatus; introducing an oxygen flow under pressure intosaid enriching chamber; subjecting said liquid to an oxygen pressure toenrich said liquid with oxygen; recovering the oxygen enriched liquid.2. A method according to claim 1, wherein said liquid is subjected to anoxygen pressure of from 8 to 14 atmospheres within said enrichingchamber.
 3. A method according to claim 2, wherein the oxygen pressureof from 8 to 14 atmosphere is maintained within said enrichment chamberfor a time of 45 to 70 minutes to achieve a stable oxygen enrichedliquid.
 4. A method according to claim 1, wherein the liquid ismaintained under conditions of high pressure to reach an oxygenconcentration of 1-3 ml O₂/g in the liquid.
 5. A method for preparing anoxygen enriched liquid comprising the steps of: contacting a liquid withoxygen under hyperbaric conditions within an oxygen enrichment chamberof a sealed pressurization apparatus; maintaining said liquid underoxygen hyperbaric pressure to dissolve the oxygen in the liquid; passingthe oxygen enriched liquid throughout capillary tubes at a speed toeliminate the generation of bubbles from the liquid; and recovering theliquid enriched with oxygen.
 6. A method according to claim 5, whereinthe oxygen is dissolved in the liquid under a partial pressure of 3 to10 Mpa.
 7. A method according to claim 5, wherein said liquid ismaintained into contact with oxygen under hyperbaric conditions until athe concentration of oxygen in the liquid is of from 1 to 3 ml O₂/g. 8.A method according to claim 5, wherein said capillary tubes in which theoxygen enriched liquid is passed have a diameter of 60 to 80μ.
 9. Amethod according to claim 5, wherein the oxygen enriched liquid ispassed thorough capillary tubes under hydrostatic pressure of 50 to 70Mpa.
 10. A method according to claim 5 wherein the oxygen enrichedliquid transits in said capillary tubes at a speed of 4 m/s.
 11. Amethod according to claim 5 wherein said oxygen enriched liquid ispassed thorough the capillary tubes at a rate to obtain a transit of 1g/min.
 12. A method according to claim 5 wherein said liquid isdrinkable water.
 13. A method according to claim 5 wherein said liquidis a solution selected from the group consisting of physiologicalsolution, dextrose solution, Ringer solution, Hartmann solution proteinrich solution PVP plasma and mixtures thereof.
 14. A drinkable oxygenenriched water obtainable according to the process of claim
 1. 15. Adrinkable oxygen enriched water obtainable according to the process ofclaim
 5. 16. A method of treatment of conditions connected with adeficiency of oxygen supply comprising the administration to a human ofan effective amount of an oxygen enriched liquid prepared according tothe method of claim
 5. 17. A cosmetic method of treatment comprising thelocal application of a cosmetically effective amount of a cosmeticcomposition including an oxygen enriched liquid prepared according tothe method of claim
 1. 18. A method of treatment bacterial infectionscomprising the administration of an antibacterial effective amount of aphysiological enriched solution prepared according to the method ofclaim 5.